Callous thieves broke into Jollies Barn in Mere Brow during the night.The break in was discovered at 6.45 this morning.
Doors were damaged and cash was stolen from the tills.
The Playbarn was also targeted in March 2014 when burglars smashed through the door and stole the safe.
Owner Sara Brookes is appealing for information after this latest burglary.
Anyone with information is asked to call Lancashire Police on 101